CLONE # :      

ynrhTNF-a.

IMMUNOGEN :           

  r.Human TNF-a.

Ig SUBCLASS :                           

Mouse IgG1.

PURIFICATION METHOD:     

Protein A (high salt).

PROTEIN CONCENTRATION:      

1 mg/ml in PBS (after reconstitution).

STORAGE PROCEDURES:  

Lyophilized: store at 4^o C.  After reconstitution, if  not intended for use within a month, aliquot and   store at ‑20^o C.

RECONSTITUTION:

Reconstitute with 0.5 ml of H₂0.  Mix gently, wash  the sides of the vial and wait 30-60  seconds before use.

STABILITY / SHELF LIFE:

Two years lyophilized, 1month in solution at 4^oC.  

TITER:

By direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson

ImmunoResearch  Laboratories) 1:10,000 dilution will yield 0.7 O.D within 

10 minutes

NOTE:

This antibody will bind very well to protein A in  a buffer (PBS) containing high

salt concentration (3M Nacl).

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