CLONE # :      

YNRhIL2

IMMUNOGEN :           

  r.Human IL-2

Ig SUBCLASS :                           

Mouse IgG2a

PURIFICATION METHOD:     

Protein A

PROTEIN CONCENTRATION:      

1 mg/ml in PBS (after reconstitution).

STORAGE PROCEDURES:  

Lyophilized: store at 4^o C.  After reconstitution, if  not intended for use within a month, aliquot and   store at ‑20^o C.

RECONSTITUTION:

Reconstitute with 0.5 ml of H₂0.  Mix gently, wash  the sides of the vial and wait 30-60  seconds before use.
 

STABILITY / SHELF LIFE:

Two years lyophilized, 1month in solution at 4^oC.  
 

TITER:

   By direct ELISA, 1:10,000 dilution will yield 0.4 O.D using alkaline    phosphatase  conjugated rabbit anti-mouse Ig (Jackson Laboratories).     1:50 dilution will block  70-80% of CTLL proliferative response to 50 units     of r.human IL-2. 

    CROSS REACTIVITY:

  A certain degree of functional cross reactivity with murine IL-2 was    reported by some investigators.

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